• Assay Type

  Solid Phase Sandwich ELISA

• Format

  96-well strip plate

• Range

  0.312 - 20 ng/mL

• Sufficient Materials

  For five 96-well plates*

• Specificity

  Please see the

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant human Matrix Metalloproteinase 9/Neutrophil Gelatinase-associated Lipocalin Complex (MMP-9/NGAL Complex). The suggested diluent is suitable for the analysis of most cell culture supernate, serum, plasma, saliva, and urine samples. Diluents for complex matrices, such as serum, plasma, saliva, and urine, should be evaluated prior to use in this DuoSet.

Product Features

• Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time

• Development protocols are provided to guide further assay optimization

• Assay can be customized to your specific needs

• Economical alternative to complete kits

Kit Content

• Capture Antibody

• Detection Antibody

• Recombinant Standard

• Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

  PBS: (Catalog # ), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na  ₂HPO  ₄, 1.5 mM KH  ₂PO  ₄, pH 7.2 - 7.4, 0.2 µm filtered  
 
  Wash Buffer: (Catalog # ), or equivalent  
 
  Reagent Diluent*  
 
  Blocking Buffer*  
 
  Substrate Solution: 1:1 mixture of Color Reagent A (H  ₂O  ₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # )  
 
  Stop Solution: 2 N H  ₂SO  ₄ (Catalog # )  
 
  Microplates: R&D Systems (Catalog # ), or equivalent  
 
  Plate Sealers: ELISA Plate Sealers (Catalog # ), or equivalent  
 
  Normal Goat Serum: R&D Systems (Catalog # ), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product.

Preparation and Storage

• Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.