• Species Reactivity

  Human

• Specificity

  Detects human Carboxypeptidase B2/CPB2 in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human (rh) Carboxypeptidase A1, A4, recombinant mouse (rm) Carboxypeptidase A1, A4, B1, and 5% cross-reactivity with rhCarboxypeptidase B1, B2, and E is observed under reducing conditions. No cross-reactivity with any of these proteins is observed under nonreducing conditions.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 650801

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase B2/CPB2    
  Phe23-Val423 (predicted)    
  Accession # Q96IY4

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

• Immunoprecipitation

  25 µg/mL

  Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase B2/CPB2, .

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carboxypeptidase B2/CPB2

CPB2 (Carboxypeptidase B2; also CPU and TAFI) is a secreted, 50-60 kDa glycoprotein member of the peptidase M14 family of enzymes.  It is expressed by hepatocytes (60 kDa) and platelets (50 kDa), with MW differences attributable to glycosylation.  CPB2 is cleaved by thrombin and plasmin, generating a 36 kDa, relatively insoluble nonglycosylated enzymatically active fragment (TAFIa).  Active CPB2 removes C-terminalresidues from fibrin, thereby interrupting plasmin generation and promoting fibrin polymerization.  Human CPB2 (proprecursor/zymogen) is 401 amino acids (aa) in length.  It contains a prosequence (aa 23-114) and an active fragment (aa 115-423) that acts on C-terminalor Arg residues.  There is one potential isoform variant that shows a deletion of aa 198-234 accompanied by a 16 aa substitution for aa 382-423.  Over aa 23-423, human CPB2 shares 85% aa identity with mouse CPB2.  

• Entrez Gene IDs:

  1361 (Human); 56373 (Mouse); 113936 (Rat)

• Alternate Names:

  carboxypeptidase B2 (plasma); carboxypeptidase B2 (plasma, carboxypeptidase U); Carboxypeptidase B2; Carboxypeptidase U; CPB2; CPU; CPUEC 3.4.17.20; EC 3.4.17; PCPB; Plasma carboxypeptidase B; TAFI; TAFIcarboxypeptidase B-like protein; Thrombin-activable fibrinolysis inhibitor; thrombin-activatable fibrinolysis inhibitor