• Species Reactivity

  Mouse

• Specificity

  Detects mouse FGF-4 in direct ELISAs. In direct ELISAs, 10% - 50% cross-reactivity with recombinant mouse (rm) FGF-10, rmFGF-7, recombinant human (rh) FGF-16, rhFGF-20 and less than 5% cross-reactivity with rmFGF acidic, rmFGF basic, rmFGF-6, -8b,  -8c, -15, -17,    
  -21, -23, rhFGF acidic, rhFGF basic, rhFGF-3, -4, -5, -9, -11, -12, -13, -18, -19, or -22 is observed.

• Source

  Monoclonal Rat IgG    _2B Clone # 678431

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant mouse FGF-4    
  Ala30-Leu202    
  Accession # NP_034332

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-4

FGF-4 (fibroblast growth factor-4), also known as FGF-K or K-FGF (Kaposi’s sarcoma-associated FGF), is a 25 kDa secreted, heparin-binding member of the FGF family (1, 2). The mouse FGF-4 cDNA encodes 202 amino acids (aa) with a 29 aa signal sequence and a 173 aa mature protein with an FGF homology domain that contains a heparin binding region near the C-terminus (2). Mature mouse FGF-4 shares 87%, 90%, 87% and 85% aa identity with human, rat, canine and bovine FGF‑4, respectively. Human FGF-4 has been shown to exhibit cross species activity. Expression of FGF-4 and its receptors, FGF R1c, 2c, 3c and 4, is spatially and temporally regulated during embryonic development (1, 3). Its expression in the trophoblast inner cell mass promotes expression of FGF R2, and is required for maintenance of the trophectoderm and primitive endoderm (3‑5). Later in development, FGF-4 works together with FGF-8 to mediate the activities of the apical ectodermal ridge, which direct the outgrowth and patterning of vertebrate limbs (3, 6‑9). FGF-4 is proposed to play a physiologically relevant role in human embryonic stem cell self-renewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in vitro (10‑12). A C-terminally truncated 15 kDa isoform that opposes full-length FGF-4 and promotes differentiation is endogenously expressed in human embryonic stem cells. FGF-4 is mitogenic for fibroblasts and endothelial cells in vitro and has autocrine transforming potential (13). It is a potent angiogenesis promoter in vivo and has been investigated as therapy for coronary artery disease (14).

• References:

1. Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139.

2. Hebert, J.M. et al. (1990) Dev. Biol. 138:454.

3. Niswander, L. and G.R. Martin (1992) Development 114:755.

4. Feldman, B. et al. (1995) Science 267:246.

5. Goldin, S.N. and V.E. Papaioannou (2003) Genesis 36:40.

6. Sun, X. et al. (2002) Nature 418:501.

7. Boulet, A.M. et al. (2004) Dev. Biol. 273:361.

8. Yu, K and D.M. Ornitz (2008) Development 135:483.

9. Mariani, F.V. et al. (2008) Nature 453:401.

10. Johannesson, M. et al. (2009) PLoS ONE 4:e4794.

11. Kunath, T. et al. (2007) Development 134:2895.

12. Mayshar, Y. et al. (2008) Stem Cells 26:767.

13. Hajitou, A. et al. (1998) Oncogene 17:2059.

14. Flynn, A. and T. O’Brien (2008) IDrugs 11:283.

• Long Name:

  Fibroblast Growth Factor 4

• Entrez Gene IDs:

  2249 (Human); 14175 (Mouse); 116499 (Rat)

• Alternate Names:

  FGF4; FGF-4; fibroblast growth factor 4; HBGF-4; HBGF-4Transforming protein KS3; heparin secretory transforming protein 1; Heparin secretory-transforming protein 1; Heparin-binding growth factor 4; HST-1; HST-1HSTF-1; HSTF1fibroblast growth factor 4 splice isoform; HSTFGF-4; human stomach cancer, transforming factor from FGF-related oncogene; kaposi sarcoma oncogene; KFGF; K-FGF; KS3; oncogene HST