• Species Reactivity

  Human

• Specificity

  Detects human Fes in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG    _2B Clone # 678527

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Fes    
  Ser270-Ala370    
  Accession # P07332

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.2 µg/mL

  See below

• 
  

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fes

Fes (homolog of feline sarcoma oncogene), also called c-FES or c-FPS, is a 92 kDa protein within subgroup IV of the non-receptor tyrosine kinase family. It is expressed in myeloid hematopoietic cells, vascular endothelium and neurons and regulates cell adhesion and extravasation. The 822 amino acid (aa) human Fes contains F-BAR (aa 7-239), SH2 (aa 460-549) and kinase (aa 561-822) domains. The F-BAR domain contains coiled-coil homo-oligomerization and microtubule binding sequences. Within the region used as an immunogen, human Fes shares 85% and 87% aa identity with mouse and rat Fes, respectively. Human isoforms of 764, 752, 694 and 681 aa may be missing aa 72-129 or 441-511 and/or contain an alternate 17 aa for aa 610-640, while an isoform of 891 aa contains 18 and 51 aa inserted after aa 510 and 568.

• Long Name:

  Feline Sarcoma Proto-oncogene

• Entrez Gene IDs:

  2242 (Human); 14159 (Mouse); 361597 (Rat)

• Alternate Names:

  EC 2.7.10; EC 2.7.10.2; feline sarcoma oncogene; feline sarcoma virus; Fes; FPS; Proto-oncogene c-Fes; Proto-oncogene c-Fps; proto-oncogene tyrosine-protein kinase Fes/Fps; tyrosine-protein kinase Fes/Fps