• Species Reactivity

  Mouse

• Specificity

  Detects mouse TEX19.1 in direct ELISAs.

• Source

  Monoclonal Rat IgG    _2A Clone # 678826

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant mouse TEX19.1    
  Arg189-Pro351    
  Accession # Q99MV2

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TEX19.1

Testis-expressed protein 19.1 (TEX19.1; also Tex19a and Tex19) is a 40 kDa (calculated) pluripotent stem cell molecule. Mouse TEX19.1 is 351 amino acids (aa) in length.  The first conserved domain is localized on the N-terminal boundary of the protein and is 58 residues long.  It is known as the MCP domain, and begins with an invariant MCPPVS motif. The second conserved domain, known as the VPTEL domain, begins with an invariant VPTEL motif and is 38 amino acids long. Mouse TEX19.1 shares 70% and 24% aa identity with rat and human Tex19.1, respectively. The protein is restricted to mammals and is expressed in testis, placenta, and ovary.  It is expressed in early embryo and is later limited to the germ line. TEX19.1 has been shown to present in the cytoplasm of spermatogonia and early spermatocytes in adult mouse testes (1). TEX19.1 is thought to be a transcription factor regulating target genes responsible for pluripotency; alternatively, it may have a role in controlling the chromatin architecture of the pluripotent nucleus.

• References:

1. Ollinger R. et. al. (2008) PLos 4(9):e1000199.

• Long Name:

  Testis Expressed 19.1

• Entrez Gene IDs:

  73679 (Mouse)

• Alternate Names:

  TEX19.1; Tex19a