• Species Reactivity

  Mouse

• Specificity

  Detects mouse CD3 epsilon. Binds to lymphocytes from all mouse strains tested and does not react with cells from rats, rabbits, miniature swine, or hamsters.    ¹ Binds to the CD3 epsilon -chain present on T-lymphocyes and thymocytes. Its binding has been characterized with respect to several other monoclonal anti-CD3 antibodies.    ^3,4  

• Source

  Monoclonal Hamster IgG Clone # 145-2C11

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  H-2K    ^b-specific mouse cytotoxic T-lymphocyte

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  2.5 µg/10    ⁶ cells

  Mouse splenocytes

• 
  

• Immunoprecipitation

  Leo, O.     et al. (1987) Proc. Natl. Acad. Sci.     84:1374.

• 
  

• Cell depletion

  Small, M.     et al. (1994) J. Immunol. Meth.     167:103.

• 
  

• CyTOF-reported

  Fienberg, H.G.     et al. (2012) Cytometry     81: 467. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• T Cell Stimulation

  0.1-0.6 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD3 epsilon

CD3 is composed of five different polypeptides ranging from 16‑28 kDa that are associated with the T cell receptor (TCR) complex and serve as its signal transducing element. The CD3/TCR complex is expressed on T cells and thymocytes. Binding of immobilized anti-CD3 can cause T cell activation that leads to any of several consequences, depending on the conditions. Activation by anti-CD3 in the presence of IL 2 has been reported to induce cell death, apparently via apoptosis.  ⁴ The antibody can be used to induce cytolytic activity against non-specific targets and also to block TCR-mediated cytolytic killing.  ¹ 145-2C11 has been used in a variety of studies concerned with allograft rejection and graft-vs host reaction in mice.  ^{6, 7}

• References:

1. Leo, O. et al. (1987) Proc. Natl. Acad. Sci. USA 84:1374.

2. Portoles, P. et al. (1989) J. Immunol. 142:4169.

3. Coulie, P.G. et al. (1991) Eur. J. Immunol. 21:1703.

4. Ucker, D.S. J. Meyers and P.S. Obermiller. (1992) J. Immunol. 149:1583.

5. Small, M. et al. (1994) J. Immunol. Meth. 167:103.

6. Alegre, M.L. et al. (1991) J. Immunol. 146:1184.

7. Hendrickson, M. et al. (1995) Transplantation 60:828.

• Entrez Gene IDs:

  916 (Human); 12503 (Mouse)

• Alternate Names:

  CD3 epsilon; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE