• Species Reactivity

  Human

• Specificity

  Detects human STAT5b in Western blots.

• Source

  Monoclonal Mouse IgG2b Clone # 389215

• Immunogen

  Human STAT5b synthetic peptide    
  MDSQWIPHAQS    
  Accession # NP_036580

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with methanol

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: STAT5b

Signal Transduction and Activator of Transcription 5B (STAT5b) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL-22, IL-6, IFN alpha ). Upon cytokine activation, STAT5b is phosphorylated and translocates to the nucleus where it binds to the DNA consensus site TTCN₃GAA and activates transcription of specific genes. STAT5b shares 93% amino acid identity with STAT5a. STAT5b is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation and survival.

• Long Name:

  Signal Transducer and Activator of Transcription 5b

• Entrez Gene IDs:

  6777 (Human); 20851 (Mouse)

• Alternate Names:

  signal transducer and activator of transcription 5B; STAT5; STAT5b; transcription factor STAT5B