• Species Reactivity

  Human

• Specificity

  Detects human ECE-1 in direct ELISAs.

• Source

  Monoclonal Rat IgG1 Clone # 303913

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human ECE-1    
  Gln90-Trp770    
  Accession # P42892

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  MCF‑7 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ECE-1

Endothelin-converting Enzyme 1 (ECE-1) is a zinc protease of the neprilysin (NEP) family, which also includes ECE-2, PEX, XCE, DINE, Kell and several NEP-like proteins (1). ECE-1 is a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Four alternatively spliced isoforms differ in their cytoplasmic tail (2, 3). In addition to big endothelin-1, ECE-1 cleaves a variety of bioactive peptides such as bradykinin, neurotensin, angiotensin I, and substance P (1). Together with ECE-2, it is also involved in degradation of beta -amyloid peptide (4). The ectodomain of human ECE-1, which is common to all isoforms, was expressed with an N-terminal His tag and purified.

• References:

1. Turner, A.J. et al. (2001) BioEssays 23:261.

2. Valdennaire, O. et al. (1999) Eur. J. Biochem. 264:341.

3. Schweizer, A. et al. (1997) Biochem. J. 328:871.

4. Eckman, E.A. et al. (2003) J. Biol. Chem. 278:2081.

• Long Name:

  Endothelin-converting Enzyme-1

• Entrez Gene IDs:

  1889 (Human)

• Alternate Names:

  EC 3.4.24; EC 3.4.24.71; ECE; ECE1; ECE-1; endothelin converting enzyme 1; endothelin-converting enzyme 1