• Species Reactivity

  Human

• Specificity

  Detects human CRISP-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CRISP-2 is observed.

• Source

  Monoclonal Mouse IgG2b Clone # 295208

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human CRISP-3    
  Asn21-Tyr245 (Ser106Pro & Ala134Ser)    
  Accession # P54108.1

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  PC‑3 human prostate cancer cell line fixed with paraformaldehyde and permeabilized with saponin

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CRISP-3

CRISP-3 is one of three CRISPs (cysteine-rich secretory proteins) found in mammalian exocrine secretions and granulocytes that may play a role in innate immunity (1‑3). CRISPs and several snake, insect, and lizard venom proteins are characterized by 16 invariant cysteine residues (4). Structurally, they consist of an N-terminal SCP domain, a hinge region, and a cysteine-rich domain (5). CRISP-3 is produced by salivary, pancreas, prostate, and lacrimal glands, as well as spermatozoa and mature spermatids (2, 6, 7). In mouse, however, CRISP-3 has not been detected in the male genital tract (8, 9). CRISP-3 is up-regulated in epithelial prostate cancer and chronic pancreatitis (10, 11). It is present as 30 kDa and 28 kDa species, corresponding to glycosylated and nonglycosylated forms (1, 3, 7, 10, 12). In serum and seminal fluid, CRISP-3 forms high affinity noncovalent complexes with the more abundant alpha 1B-glycoprotein and beta -microseminoprotein/PSP94, respectively (12, 13). Binding is mediated by the SCP domain of CRISP-3 and is independent of glycosylation (12). CRISP-3 is also expressed in pre-B cells but not in T cells or monocytes (14, 15). CRISP-3 is released from neutrophil and eosinophil granules following cell stimulation (1, 15). Mature human CRISP-3 shares 48% and 65% amino acid (aa) sequence identity with mouse and equine CRISP-3, respectively. It shares 44% and 72% aa sequence identity with human CRISP-1 and -2, respectively.

• References:

1. Kjeldsen, L. et al. (1996) FEBS Lett. 380:246.

2. Kratzschmar, J. et al. (1996) Eur. J. Biochem. 236:827.

3. Udby, L. et al. (2002) J. Immunol. Meth. 263:43.

4. Yamazaki, Y. and Morita, T. (2004) Toxicon 44:227.

5. Guo, M. et al. (2005) J. Biol. Chem. 280:12405.

6. Haendler, B. et al. (1999) J. Cell. Physiol. 178:371.

7. Udby, L. et al. (2005) J. Androl. 26:333.

8. Haendler, B. et al. (1993) Endocrinology 133:192.

9. Haendler, B. et al. (1997) Eur. J. Biochem. 250:440.

10. Bjartell, A. et al. (2006) Prostate 66:591.

11. Liao, Q. et al. (2003) Histol. Histopathol. 18:245.

12. Udby, L. et al. (2005) Biochem. Biophys. Res. Commun. 333:555.

13. Udby, L. et al. (2004) Biochemistry 43:12877.

14. Pfisterer, P. et al. (1996) Mol. Cell. Biol. 16:6160.

15. Udby, L. et al. (2002) J. Leukoc. Biol. 72:462.

• Long Name:

  Cysteine-rich Secretory Protein 3

• Entrez Gene IDs:

  10321 (Human); 11572 (Mouse)

• Alternate Names:

  Aeg2; CRISP3; CRISP-3; CRISP-3MGC126588; CRS3; cysteine-rich secretory protein 3; cysteine-rich secretory protein-3; dJ442L6.3; SGP28; SGP28Aeg2; specific granule protein (28 kDa); Specific granule protein of 28 kDa