• Species Reactivity

  Human

• Specificity

  Detects human Insulysin/IDE in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG1 Clone # 334501

• Immunogen

  S. frugiperda insect ovarian cell line     Sf 21-derived recombinant human Insulysin/IDE    
  Met42-Leu1019    
  Accession # P14735

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  HeLa cells fixed with paraformaldehyde and permeabilized with saponin

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Insulysin/IDE

Insulysin, or insulin-degrading enzyme (IDE), is a zinc metallopeptidase of the inverzincin family. IDE is primarily located in the cytosol, but has been detected as a secreted enzyme and associated with the plasma membrane as well (1). The enzyme is expressed in many tissues, with the highest levels in liver, kidney, brain, and testis (2). IDE hydrolyzes a variety of regulatory peptides, including insulin, glucagon, atrial natriuretic factor, and transforming growth factor-alpha in vitro (1). In addition, IDE has been shown to degrade the amyloid beta (A beta ) peptide, which polymerizes into the plaques associated with Alzheimer's disease (3). Deficiencies in IDE activity may contribute to the pathogenesis of type 2 diabetes mellitus (DM2) and Alzheimer's disease. The IDE region of human chromosome 10q has been genetically linked to DM2 (4). When the IDE gene was specifically disrupted in mice, IDE -/- animals developed hyperinsulinemia and glucose intolerance, characteristics of DM2 (5). The IDE -/- mice were also shown to have a significant decrease in A beta degradation in the brain, resulting in increased cerebral accumulation of A beta peptide. This in vivo evidence is consistent with the hypotheses that IDE is important for the degradation of insulin in cells and for the clearance of A beta peptide in the brain.

• References:

1. Affholter, J. A. et al. (1988) Science 242:1415.

2. Duckworth, W.C. et al. (1998) Endocr. Rev. 19:608.

3. Akiyama, H. et al. (1990) Biochem. Biophys. Res. Commun. 170:1325.

4. Selkoe, D.J. (2001) Neuron 32:177.

5. Ghosh, S. et al. (2000) Am. J. Hum. Genet. 67:1174.

6. Farris, W. et al. (2003) Proc. Natl. Acad. Sci. USA 100:4162.

• Long Name:

  Insulin Degrading Enzyme

• Entrez Gene IDs:

  3416 (Human); 15925 (Mouse); 25700 (Rat)

• Alternate Names:

  Abeta-degrading protease; EC 3.4.24; EC 3.4.24.56; FLJ35968; IDE; INSDEGM; Insulin protease; Insulinase; insulin-degrading enzyme; Insulysin