• Species Reactivity

  Human

• Specificity

  Detects human LAIR2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human LAIR1, recombinant mouse (rm) LAIR1 or rmLAIR2 is observed.

• Source

  Monoclonal Mouse IgG2b Clone # 319701

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human LAIR2 isoform 1    
  Gln22-Pro152    
  Accession # Q6ISS4

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with saponin

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: LAIR2

LAIR2 (leukocyte-associated Ig-like receptor-2; CD306) is a secreted, 131 amino acid (aa) protein that contains one Ig-like C2 type domain, making it a member of the Ig superfamily. When compared to LAIR1, its transmembrane counterpart, it shares 83% aa identity across the signal sequence and extracellular domains (1‑3). Although one is secreted and one is membrane-bound, the two LAIR proteins are thought to have arisen from a common gene ancestor and appear to share similar adhesion profiles. This suggests that LAIR2 may compete with LAIR1 for ligand binding (3, 4). A 114 aa alternate splice form of LAIR2 is truncated at the C terminus, but retains the entire Ig domain (1‑3). The expression profile of these splice forms, and the presence of orthologs in other species, have not been reported.

• References:

1. Meyaard, L. (2003) J. Biol. Regul. Homeost. Agents 17:330.

2. Meyaard, L. et al. (1999) J. Immunol. 162:5800.

3. Meyaard, L. et al. (1997)  Immunity 7:283.

4. Xu, X.G. et al. (2005) Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 21:553.

• Long Name:

  Leukocyte-associated Ig-like Receptor 2

• Entrez Gene IDs:

  3904 (Human)

• Alternate Names:

  CD306 antigen; CD306; CD306leukocyte-associated Ig-like receptor-2; LAIR2; LAIR-2; leukocyte-associated Ig-like receptor 2; leukocyte-associated immunoglobulin-like receptor 2; MGC71634