• Species Reactivity

  Human

• Specificity

  Detects human LINGO-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human LINGO-1 is observed.

• Source

  Monoclonal Mouse IgG2a Clone # 382007

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human LINGO-2    
  Cys28-Leu542    
  Accession # Q7L985

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  A549 human lung carcinoma cell line and and HEK293 human embryonic kidney cell line either transfected with human LINGO-2 and eGFP

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: LINGO-2

Human LINGO-2 (LRR and Ig domain-containing, Nogo Receptor-interacting protein 2; also known as Leucine-rich repeat neuronal 6C (LRRN6C) or LERN3), type I transmembrane protein in the neuronal leucine-rich repeat family. These proteins have a signal peptide, 12 extracellular leucine-rich repeats flanked by N-terminal and C-terminal cysteine-rich sequences, an immunoglobulin-like domain, a transmembrane domain and a short cytoplasmic tail.  An alternate start site may exist at Met148 of the precursor. Human LINGO-2 is a highly conserved, 606 amino acid protein that shares 99% and 98% aa sequence identity with canine and mouse LINGO-2, respectively. LINGO-2 presumably functions outside the CNS with little involvement by p75/NgR1.

• Long Name:

  Leucine-rich Repeat And Ig Domain Containing Nogo Receptor-Interacting Protein 2

• Entrez Gene IDs:

  158038 (Human); 242384 (Mouse); 313156 (Rat)

• Alternate Names:

  LERN3; LERN3FLJ31810; leucine rich repeat and Ig domain containing 2; leucine-rich repeat and immunoglobulin-like domain-containing nogoreceptor-interacting protein 2; Leucine-rich repeat neuronal protein 3; Leucine-rich repeat neuronal protein 6C; LINGO2; LINGO-2; LRRN6C; LRRN6Cleucine rich repeat neuronal 6C