• Species Reactivity

  Human

• Specificity

  Detects human Claudin‑10b. Stains human Claudin‑10b transfectants but not irrelevant transfectants.

• Source

  Monoclonal Mouse IgG2b Clone # 394513

• Immunogen

  NS0 mouse myeloma cell line transfected with human Claudin‑10 b Isoform    
  Met1-Val228    
  Accession # P78369

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  HepG2 human hepatocellular carcinoma cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Claudin-10b

Claudin-10 is a 228 amino acid multipass membrane protein in the Claudin family of epithelial tight junction proteins. Two splice forms of human Claudin-10 have been described with a divergent N-terminal extracellular loop. Claudin-10b (Swiss-Prot isoform 1) is relatively cation-permeant compared to Claudin-10a and is widely expressed, while Claudin‑10a is restricted to the kidney cortex. Claudin-10 is overexpressed in some carcinomas. Human Claudin‑10b shares 94% aa sequence identity with mouse and rat Claudin-10.

• Entrez Gene IDs:

  9071 (Human); 58187 (Mouse); 290485 (Rat)

• Alternate Names:

  Claudin10b; Claudin-10b; CLDN10; CPETRL3; OSP-L