• Species Reactivity

  Human

• Specificity

  Detects human Claudin-12. Stains human Claudin-12 transfectants but not irrelevant transfectants.

• Source

  Monoclonal Mouse IgG2a Clone # 393314

• Immunogen

  HEK293 human embryonic kidney cell line transfected with human Claudin-12    
  Met1-Thr244    
  Accession # NP_036261

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Caco‑2 human colorectal adenocarcinoma cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Claudin-12

Claudin‑12 is a 244 amino acid multipass membrane protein in the claudin family of epithelial tight junction proteins. Claudin‑12 is expressed on intestinal, bladder, and blood‑brain barrier epithelial cells. Human Claudin‑12 shares 92% aa sequence identity with mouse and rat Claudin‑12.

• Entrez Gene IDs:

  9069 (Human); 64945 (Mouse); 312054 (Rat)

• Alternate Names:

  claudin 12; Claudin12; Claudin-12; CLDN12