• Species Reactivity

  Mouse

• Specificity

  Detects mouse Contactin‑3 in direct ELISAS. In direct ELISAs, 100% cross‑reactivity with recombinant human (rh) Contactin-3 and no cross‑reactivity with recombinant mouse (rm) Contactin-1, -4, -6, rhContactin-2 or -5 is observed.

• Source

  Monoclonal Rat IgG2a Clone # 524518

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant mouse Contactin‑3    
  Glu20-Thr1003    
  Accession # Q07409

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  bEnd.3 mouse endothelioma cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Contactin-3

Contactin-3 (also BIG-1 in rat and PANG in mouse) is a 150 kDa member of the TAG/F3 subgroup, contactin family, Ig superfamily hierarchy. It is a GPI-linked glycoprotein that is expressed on cerebellar Purkinje cells, amygdaloid and thalamic neurons, and olfactory granule cells. It likely mediates cell-cell interaction and may promote neurite outgrowth. The mouse Contactin-3 preproprecursor is 1028 amino acids (aa) in length. It contains a 19 aa signal sequence, a 983 aa mature region, and a 26 aa cleavable C-terminal prosegment. The mature region shows six C2- type IG-like domains (aa 32-593), followed by four Fibronectin type III modules (aa 598-992). There is one potential alternate start site at Met128. Over aa 26-992, mouse Contactin-3 shares 92% and 96% aa identity with human and rat Contactin-3, respectively.

• Entrez Gene IDs:

  5067 (Human); 18488 (Mouse); 54279 (Rat)

• Alternate Names:

  BIG-1; CNTN3; contactin 3 (plasmacytoma associated); Contactin3; Contactin-3; PANG; PCS