• Species Reactivity

  Human

• Specificity

  Detects human CLEC-2A in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CLEC-2D, rhCLEC-2, or rhCD69 is observed.

• Source

  Monoclonal Mouse IgG1 Clone # 705518

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human CLEC-2A    
  Trp49-Leu174 (predicted)    
  Accession # Q6UVW9

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  U937 human histiocytic lymphoma cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CLEC-2A

CLEC-2A (C-type lectin domain family 2 member A), also known as PILAR and KACL, is an approximately 35 kDa type II transmembrane glycoprotein in the C-type lectin superfamily. It contains one C-type lectin domain in its extracellular region. Alternate splicing of human CLEC-2A generates an additional isoform with a substitution of 37 amino acids (aa) at the C-terminus. CLEC-2A is upregulated on antigen-activated CD8  ⁺ T cells and enhances T cell proliferation through interactions with CD161. It is also expressed on keratinocytes and binds to NKp65/KLRF2, triggering NK cell activation.

• Long Name:

  C-type Lectin Domain Family 2, Member A

• Entrez Gene IDs:

  387836 (Human)

• Alternate Names:

  CLEC2A C-type lectin domain family 2, member A ; CLEC2A; CLEC-2A; KACL; PILAR; UNQ5792