• Species Reactivity

  Human

• Specificity

  Detects human Lgr4/GPR48 in Lgr4-transfected cell-based ELISA. Stains human Lgr4/GPR48 transfected but not irrelevant transfected cells in flow cytometry.    
   

• Source

  Monoclonal Mouse IgG2b Clone # 852229

• Immunogen

  NS0 mouse myeloma cell line transfected with human Lgr4/GPR48    
  Met1-Asp951    
  Accession # Q9BXB1

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  HEK293 human embryonic kidney cell line transfected with human Lgr4/GPR48 and eGFP

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Lgr4/GPR48

Lgr4 (Leucine-rich repeat GPR 4), also known as GPR48 (G-Protein-coupled Receptor 48), is a seven-transmembrane glycoprotein receptor in the Lgr family of cell surface receptors (1, 2). While this family includes receptors for hormones such as LH, FSH, TSH, and HCG, the subfamily comprising Lgr4, Lgr5, and Lgr6 are G-protein-independent mediators of the potentiating effect of R-Spondins on Wnt signaling (1-6). Lgr4 binds and forms complexes with R-Spondins, Frizzled Wnt receptors and LRP Wnt co-receptors (5). It acts at least in part by enhancing Wnt-dependent LRP phosphorylation, internalization of LRPs, and accumulation of beta -catenin (3, 4). Human Lgr4 cDNA encodes 951 amino acids (aa), including a long N-terminal Extracellular Domain (ECD, aa 25-544) with 16-17 LRR domains that mediate ligand interaction (1). The LRR-containing ECD of human Lgr4 shares 93% aa sequence identity with mouse, rat and bovine Lgr4, and 50-60% aa identity with human Lgr5 and Lgr6. Lgr4 is widely expressed in both embryo and adult. Expression of Lgr4 mRNA in adult humans is highest in pancreas, followed by liver, heart, muscle, brain, and placenta (1). In rodents, embryonic and adult expression includes liver, kidney, adrenals, bone/cartilage, and heart (2, 7-9). Lgr4 deletion in the mouse affects development in areas of expression, for example, inhibiting fetal liver definitive erythropoiesis (9). Deletion of Lgr4 specifically from stem and progenitor cells in intestinal crypts induces loss of crypts due to insufficient Wnt signaling (5, 6). Lgr4 may be over-expressed in carcinomas and may promote invasiveness and metastasis by down-regulating p27^Kip1 expression (10).

• References:

1. Loh, E.D. et al. (2001) Biochem. Biophys. Res. Commun. 282:757.

2. Hsu, S.Y. et al. (1998) Mol. Endocrinol. 12:1830.

3. Carmon, K.S. et al. (2011) Proc. Natl. Acad. Sci. USA 108:11452.

4. Glinka, A. et al. (2011) EMBO Rep. 12:1055.

5. de Lau, W. et al. (2011) Nature 476:293.

6. Ruffner, H. et al. (2012) PLoS ONE 7:e40975.

7. Van Schoore, G. et al. (2005) Histochem. Cell Biol. 124:35.

8. Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:2241.

9. Song, H. et al. (2008) J. Biol. Chem. 283:36687.

10. Gao, Y. et al. (2006) Cancer Res. 66:11623.

• Long Name:

  Leucine-rich Repeat Containing G Protein-coupled Receptor 4

• Entrez Gene IDs:

  55366 (Human); 107515 (Mouse); 286994 (Rat)

• Alternate Names:

  GPR48; GPR48G protein-coupled receptor 48; G-protein coupled receptor 48; leucine-rich repeat containing G protein-coupled receptor 4; Lgr4