• Species Reactivity

  Human

• Specificity

  Detects human Endoglycan/PODXL2 in direct ELISAs.

• Source

  Monoclonal Mouse IgG2a Clone # 211816

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Endoglycan/PODXL2    
  Gly33-Thr500    
  Accession # Q9NZ53

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 405

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  HUVEC human umbilical vein endothelial cells

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Endoglycan/PODXL2

Endoglycan, also named Podocalyxin-like 2 protein, is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. It is expressed in hematopoietic precursors, endothelial cells, smooth muscle cells and leukocyte subpopulations. Endoglycan is a potential marker for hematopoietic progenitors. It also functions as an L-Selectin ligand and may be involved in the inhibition of stem cell differentiation and leukocyte-endothelial adhesion (1, 2).

• References:

1. Sassetti, C. et al. (2000) J. Biol. Chem. 275:9001.

2. Fieger, C.B. et al. (2003) J. Biol. Chem. 278:27390.

• Entrez Gene IDs:

  50512 (Human); 319655 (Mouse)

• Alternate Names:

  Endoglycan; PODLX2; podocalyxin-like 2; podocalyxin-like protein 2; PODXL2