• Species Reactivity

  Human

• Specificity

  Detects human GPR50. Stains human GPR50 transfectants but not irrelevant transfectants.

• Source

  Monoclonal Mouse IgG2a Clone # 461129

• Immunogen

  NS0 mouse myeloma cell line transfected with human GPR50    
  Met1-Val617    
  Accession # Q13585

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 405

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GPR50

GPR50, also known as MTR1L, is a non-glycosylated seven-transmembrane G protein-coupled receptor that is related to the melatonin receptors MT1 and MT2. GPR50 is expressed in the hippocampus, hypothalamus, and pituitary and forms 130 kDa homodimers. It heterodimerizes with either MT1 or MT2, resulting in inhibition of MT1 but not MT2 function. An alternately spliced isoform of GPR50 has a 4 aa deletion in the large C-terminal cytoplasmic domain. The presence of this deletion as well as various polymorphisms have been associated with elevated serum triglyceride and HDL levels. The deletion may also be associated with the development of bipolar disorder. Human GPR50 shares approximately 70% amino acid sequence identity with mouse and rat GPR50.

• Long Name:

  G Protein-coupled Receptor 50

• Entrez Gene IDs:

  9248 (Human); 14765 (Mouse); 117097 (Rat)

• Alternate Names:

  G protein-coupled receptor 50H9Mel1c; GPR50; H9; melatonin-related receptor; MGC125342; MTR1L