• Species Reactivity

  Human

• Specificity

  Detects human FCRLB/FCRY in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG2b Clone # 454217

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human FCRLB/FCRY    
  Ala18-Ser426    
  Accession # Q6BAA4

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Daudi human Burkitt's lymphoma cell line fixed with paraformaldehyde and permeabilized with methanol

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FCRLB/FCRY

FCRLB, also known as FCRL2, FCRY, and FREB2, is a 60 kDa protein with sequence homology to classical Fc receptors. There are at least six type 1 transmembrane FCRL proteins and two that lack transmembrane segments. Each family member contains between three and nine immunoglobulin-like domains. FCRL proteins are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRLB binds to purified human IgG with high affinity. Human FCRLB shares 82% and 85% amino acid sequence identity with mouse and rat FCRLB, respectively. It contains a putative signal peptide, three immunoglobulin-like domains, and a mucin-like stalk that is rich in Pro, Ser, and Thr residues (1‑3). The stalk region also contains di-Leu motifs and an unpaired cysteine (1‑3). Alternative splicing generates isoforms with deletions in the putative signal peptide and substitutions and/or truncations in the third Ig-like domain (3). When expressed in transfectants, FCRLB is not secreted but shows a diffuse intracellular localization (3, 4). FCRLB is expressed at a low level in placenta and in B lineage cells of the germinal center (3, 4). It is upregulated in B cells by BAFF and LPS (5). Among non-hematopoietic cells, FCRLB is expressed in fibroblasts, melanocytes, and melanoma (3, 5). It is preferentially expressed in nonproliferating cells and at the onset of apoptosis (4, 5).

• References:

1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.

2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.

3. Chikaev, N.A. et al. (2005) Genomics 85:264.

4. Wilson, T.J. and M. Colonna (2005) Genes Immun. 6:341.

5. Masuda, K. et al. (2005) Gene 363:32.

• Long Name:

  Fc Receptor-like B

• Entrez Gene IDs:

  127943 (Human); 435653 (Mouse); 498275 (Rat)

• Alternate Names:

  Fc receptor homolog expressed in B cells protein 2; Fc receptor like 2; Fc receptor-like and mucin-like 2; Fc receptor-like and mucin-like protein 2; Fc receptor-like B; Fc receptor-like protein 2; Fc receptor-related protein Y; FcRL2; FCRL2FCRLM2; FCRLB; FCRLM2; FCRLY; FCRY; FLJ31052; FREB2; FREB-2; FREB-2FcRY; RP11-474I16.6