• Species Reactivity

  Human

• Specificity

  Detects human TRIM in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG1 Clone # 488806

• Immunogen

  E. coli-derived recombinant human TRIM    
  Thr50-Asn186    
  Accession # Q6PIZ9

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Human blood‑derived CD3    ⁺ lymphocytes fixed with paraformaldehyde and permeabilized with saponin

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TRIM

Human TRIM (T cell receptor-interacting molecule) (also trat1, T cell receptor-associated transmembrane adaptor 1, and pp29/30) is a 30 kDa, type III transmembrane protein, that is a member of the transmembrane adaptor protein (TRAP) family. It contains a short, 8 aa extracellular region, a 19 aa transmembrane region, and a 159 aa cytosolic tail. Its cytoplasmic tail contains several tyrosine motifs with the potential to bind to Src-homology 2 (SH2) domains of signaling proteins. TRIM is present in T cells and NK cells. Human TRIM shares 66% aa sequence identity with mouse TRIM.

• Long Name:

  T Cell Receptor Interacting Molecule

• Entrez Gene IDs:

  50852 (Human); 77647 (Mouse); 498075 (Rat)

• Alternate Names:

  pp29/30; T cell receptor associated transmembrane adaptor 1; T cell receptor interacting molecule; T-cell receptor interacting molecule; T-cell receptor-associated transmembrane adapter 1; T-cell receptor-interacting molecule; TCRIM; TRAT1; TRIM; TRIMHSPC062