• Species Reactivity

  Human

• Specificity

  Detects human MRGX2. Stains human MRGX2 transfectants but not irrelevant transfectants.

• Source

  Monoclonal Mouse IgG    _2A Clone # 477533

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  NS0 mouse myeloma cell line transfected with human MRGX2    
  Met1-Val330    
  Accession # Q96LB1

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 647

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MRGX2

MRGX2 (MAS-related GPR, member X2) is a 330 amino acid (aa) G-protein coupled 7-transmembrane protein that is selectively expressed in small-diameter sensory neurons of dorsal root ganglia. Human MRGX shares 41‑52% aa identity with three other primate MRGX proteins, but has no ortholog in rodents. Binding of ligands such as cortistatin, proadrenomedullin peptides (PAMP-12 and –20) and basic peptides (substance P, neuropeptide Y) to MRGX2 can activate Gq or Gi regulated pathways. MRGX2 is thought to influence nociception and promote adrenal gland catecholamine secretion and IgE-independent mast cell degranulation.

• Long Name:

  Mas-related G Protein-coupled Receptor Member X2

• Entrez Gene IDs:

  117194 (Human); 243978 (Mouse)

• Alternate Names:

  MAS-related GPR, member X2; mas-related G-protein coupled receptor member X2; MRGPRX2; MRGX2; MRGX2G protein-coupled receptor MRGX2