• Species Reactivity

  Human

• Specificity

  Detects human AATK in direct ELISAs.

• Source

  Monoclonal Mouse IgG2b Clone # 456006

• Immunogen

  E. coli-derived recombinant human AATK    
  Asn421-Ala589    
  Accession # AAH47378

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: AATK

Apoptosis-associated tyrosine kinase (AATK) is a 155 kDa membrane-associated intracellular protein that promotes neurite extension and neuronal differentiation. Low extracellular potassium levels induce AATK phosphorylation and apoptosis in cerebellar granule cells. AATK negatively regulates the Na-K-2Cl cotransporter by promoting its PP1-dependent dephosphorylation. The two alternate splice forms of AATK also contain the sequences included in this immunogen. Within this region, human AATK shares 63% aa sequence identity with mouse AATK.

• Long Name:

  Apoptosis-associated Tyrosine Kinase

• Entrez Gene IDs:

  9625 (Human); 11302 (Mouse); 690853 (Rat)

• Alternate Names:

  AATK; AATYK; AATYKp35-binding protein; apoptosis-associated tyrosine kinaseCDK5-binding protein; Brain apoptosis-associated tyrosine kinase; EC 2.7.11; KIAA0641p35BP; Lemur tyrosine kinase 1; LMR1; LMR1EC 2.7.11.1; LMTK1; LMTK1AATYK1; p35BP; serine/threonine-protein kinase LMTK1