• Species Reactivity

  Human

• Specificity

  Detects human TAFA4/FAM19A4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human TAFA1, 2, 3, or 5 is observed.

• Source

  Monoclonal Mouse IgG1 Clone # 480103

• Immunogen

  E. coli-derived recombinant human TAFA4    
  Ser35-Arg140    
  Accession # Q96LR4

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TAFA4/FAM19A4

TAFA4 (also FAM19A4) is a secreted, 12 kDa member of the FAM19/TAFA family of chemokine-like proteins (1). It is synthesized as a 140 amino acid (aa) precursor that contains a 35 aa signal sequence and a 105 aa mature chain. Like other members of the FAM19/TAFA family, with the exception of TAFA5, mature TAFA1 contains 10 regularly spaced cysteine residues that follow the pattern Cx₇CCx₁₃CxCx₁₄Cx₁₁Cx₄Cx₅Cx₁₀C, where C represents a conserved cysteine residue and x represents any noncysteine amino acid (1). Human TAFA4 is 90% aa identical to mouse TAFA4 (1). Real-time PCR analysis indicates that TAFA4 mRNA expression is restricted to the central nervous system (CNS), with the highest level in the thalamus (1). TAFAs may modulate immune responses in the CNS by functioning as brain specific chemokines and may act with other chemokines to optimize the recruitment and activity of immune cells in the CNS (1). TAFAs may represent a novel class of neurokines that act as regulators of immune nervous cells (1, 2). TAFAs may also control axonal sprouting following brain injury (1).

• References:

1. Tang, Y.T. et al. (2004) Genomics 83:727.

2. Benveniste, E. (1998) Cytokine Growth Factor Rev. 9:259.

• Long Name:

  Family with Sequence Similarity 19, Member A4

• Entrez Gene IDs:

  151647 (Human); 320701 (Mouse)

• Alternate Names:

  Chemokine-like protein TAFA-4; FAM19A4; family with sequence similarity 19 (chemokine (C-C motif)-like), member A4; FLJ25161; TAFA4; TAFA-4