• Species Reactivity

  Human

• Specificity

  Stains human GPRC5A transfectants but not irrelevant transfectants.

• Source

  Monoclonal Mouse IgG2a Clone # 481906

• Immunogen

  NS0 mouse myeloma cell line transfected with human GPRC5A    
  Met1-Ser357    
  Accession # Q8NFJ5

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  A549 human lung carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GPRC5A

G-protein coupled receptor family C group 5 member A (GPRC5A), also known as Retinoic acid-induced gene 1 protein (RAIG-1), is a member of the G-protein coupled receptor 3 family. It has a molecular weight of approximately 35 kDa and contains seven transmembrane domains. Human GPRC5A shares 76% and 74% aa sequence identity with mouse and rat GPRC5A, respectively. It is expressed at high levels in fetal and adult lung and is also present in fetal kidney and adult placenta, kidney, prostate, testis, ovary, small intestine, colon, stomach, and spinal chord at lower levels.

• Long Name:

  G Protein-coupled Receptor, Family C, Group 5, Member A

• Entrez Gene IDs:

  9052 (Human); 232431 (Mouse); 312790 (Rat)

• Alternate Names:

  G protein-coupled receptor, family C, group 5, member A; GPCR5A; GPRC5A; Orphan G-protein-coupling receptor PEIG-1; RAI3; RAI3G-protein coupled receptor family C group 5 member A; RAIG1; RAIG1RAIG-1; retinoic acid induced 3; retinoic acid responsive; Retinoic acid-induced gene 1 protein; retinoic acid-induced protein 3