• Purity

  >90%, by SDS-PAGE with silver staining

• Endotoxin Level

  <1.0 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit serum deprivation induced apoptosis in HUVEC human umbilical vein endothelial cells. Kwak, H.J.     et al. (1999) FEBS Letters     448:249. The ED    ₅₀ for this effect is 10-40 ng/mL in the presence of 5 μg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # ).

• Source

  Mouse myeloma cell line, NS0-derived Ser20-Phe498, with a C-terminal 6-His tag Accession # Q5HYA0

• Accession #

• N-terminal Sequence    
  Analysis

  Ser20

• Structure / Form

  Oligomer

• Predicted Molecular Mass

  56 kDa  (unlabeled)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Angiopoietin-1

Angiopoietin-1 (Ang-1) is a secreted glycoprotein that plays a critical role in the development and maintenance of the vascular system (1, 2). It contains a N-terminal coiled-coil region and a C-terminal fibrinogen-like domain separated by a short flexible region (3, 4). Mature human Angiopoietin-1 shares 97% amino acid sequence identity with mouse and rat Angiopoietin-1. It is expressed by vascular smooth muscle cells and pericytes as an approximately 70 kDa molecule that associates into  
disulfide-linked homotrimers, tetramers, and pentamers (3, 5). Angiopoietin-1 binds and activates the receptor tyrosine kinase Tie-2, and its association into tetramers is important for full Tie-2 activation (3, 4). Angiopoietin-1 ligation of Tie-2 on vascular endothelial cells (EC) induces the development and branching of blood vessels (6, 7). In sub-confluent EC (  i.e. during angiogenesis), Angiopoietin-1 promotes EC motility and Tie-2 localization at the trailing edge of the cell (8). In confluent EC (  i.e. in homeostasis), multimeric Angiopoietin-1 enhances vascular integrity by promoting the   in trans homotypic association of Tie-2 between EC or with the substratum (8, 9). In addition, Angiopoietin-1 suppresses several VEGF-induced effects on the vasculature including endothelial permeability, stretch-induced release of Angiopoietin-2, and up-regulation of the leukocyte adhesion molecules VCAM-1, ICAM-1, and E-Selectin (10-12). Angiopoietin-1 also interacts with a variety of integrins and the extracellular matrix independently of Tie-2 (13, 14). These interactions support the adhesion, migration and stress resistance of EC, fibroblasts, and myocytes (13, 14). Angiopoietin-1 can protect against pulmonary arterial hypertension (5), reduce the extent of fibrosis and remodeling in infarcted diabetic myocardium (15), and enhance tumor progression and metastasis (16).

• References:

1. Koh, G.Y. (2012) Trends Mol. Med. 19:31.

2. Suri, C. et al. (1996) Cell 87:1171.

3. Davis, S. et al. (1996) Cell 87:1161.

4. Kim, K.-T. et al. (2005) J. Biol. Chem. 280:20126.

5. Kugathasan, L. et al. (2009) J. Exp. Med. 206:2221.

6. Suri, C. et al. (1998) Science 282:468.

7. Jeansson, M. et al. (2011) J. Clin. Invest. 121:2278.

8. Saharinen, P. et al. (2008) Nat. Cell. Biol. 10:527.

9. Fukuhara, S. et al. (2008) Nat. Cell Biol. 10:513.

10. Jho, D. et al. (2005) Circ. Res. 96:1282.

11. Korff, T. et al. (2012) Cardiovasc. Res. 94:510.

12. Kim, I. et al. (2001) Circ. Res. 89:477.

13. Carlson, T.R. et al. (2001) J. Biol. Chem. 276:26516.

14. Dallabrida, S.M. et al. (2005) Cardiovasc. Res. 96:e8.

15. Samuel, S.M. et al. (2010) Diabetes 59:51.

16. Holopainen, T. et al. (2009) Cancer Res. 69:4656.

• Entrez Gene IDs:

  284 (Human); 11600 (Mouse)

• Alternate Names:

  AGP1; AGPT; Ang1; ANG-1; angiopoietin 1; Angiopoietin-1; ANGPT1; KIAA0003