• Species Reactivity

  Human

• Specificity

  Detects human Cyclophilin A in direct ELISAs and Western blots. Detects mouse and rat Cyclophilin in Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) Cyclophilin E, less than 15% cross-reactivity with rhCyclophilin F, and less than 5% cross-reactivity with rhCyclophilin A-like 4 is observed.

• Source

  Monoclonal Rat IgG    _2A Clone # 817815

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Cyclophilin A    
  Met1-Glu165    
  Accession # P62937

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  See below

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cyclophilin A

Cyclophilin A, also called Peptidyl-prolyl Isomerase A, PPIA, CYPA, and CYPH, was originally characterized for its ability to catalyze the transition between cis- and trans- proline residues critical for proper folding of proteins (1). Cyclophilin is also incorporated into many viruses, including HIV-1, where it has been speculated to be involved in functions such as viral assembly and infectivity (2). The immunosuppressive activity of cyclosporins has been correlated with their ability to form complexes with cyclophilins that inhibit calcineurin phosphatase activity (3) and prevent incorporation of cyclophilin into viral particles (4). The cyclosporin/cyclophilin complex selectively binds and inactivates calcineurin (3, 5), making it a useful inhibitor for studying calcineurin activity.

• References:

1. Hamilton, G.S. and J.P. Steiner  (1998) J. Med. Chem. 41:5119.

2. Cantin, R. et al. (2005) J. Virology 79:6577.

3. Liu, J. et al. (1992) Biochemistry 31:3896.

4. Wiegers K. and H.G. Krausslich (2002) Virology 294:289.

5. Liu, J. et al. (1991) Cell 66:807.

• Entrez Gene IDs:

  5478 (Human); 268373 (Mouse); 25518 (Rat)

• Alternate Names:

  Cyclophilin A; Cyclosporin A-binding protein; CYPA; CYPAMGC12404; CYPH; EC 5.2.1.8; MGC117158; MGC23397; peptidyl-prolyl cis-trans isomerase A; peptidylprolyl isomerase A (cyclophilin A); PPIA; PPIase A; Rotamase A; Rotamase; T cell cyclophilin