• Species Reactivity

  Human

• Specificity

  Detects human Desmoplakin in direct ELISAs and Western blots.    
   

• Source

  Monoclonal Mouse IgG    ₁ Clone # 824038

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Desmoplakin    
  Asn1796-Val2046    
  Accession # P15924

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.5 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Desmoplakin

DSP (desmoplakin) is the major high molecular weight protein of desmosomes, the most common type of intercellular junction in vertebrate epithelial cells.  DSP is involved in the organization of the desmosomal cadherin-plakoglobin complex, as well as in the anchoring of intermediate filaments to desmosomes.  These functions are thought to impart tensile strength and integrity to epithelial and vascular tissues.  Mutations in DSP result in several clinical phenotypes, ranging from mild skin conditions, to severe skin blistering with subsequent dehydration, to lethal heart defects.  Two major desmoplakin isoforms, DSPI and DSPII, are encoded by alternative mRNA transcripts differentially spliced from the same gene.  Human DSPI is 2,871 amino acids (aa) in length, while DSPII lacks part of a  
central alpha-helical rod domain, and is 599 aa shorter.

• Entrez Gene IDs:

  1832 (Human); 109620 (Mouse); 306871 (Rat)

• Alternate Names:

  250/210 kDa paraneoplastic pemphigus antigen; DCWHKTA; desmoplakin (DPI, DPII); desmoplakin I; desmoplakin II; Desmoplakin; DP; DPI; DPII; DSP; KPPS2; PPKS2