• Species Reactivity

  Mouse

• Specificity

  Detects mouse ETV2/ER71 in direct ELISAs.

• Source

  Monoclonal Rat IgG    _2A Clone # 827940

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant mouse ETV2/ER71    
  Asn125-Arg231    
  Accession # P41163

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ETV2/ER71

ETS translocation variant 2 (ETV2), also known as ETSRP and ER71, is a 37 kDa transcription factor that is expressed in presumptive vascular progenitor cells. It plays an important role in vascular development by promoting the expansion of hemangioblasts and vascular endothelial cells while inhibiting differentiation into cardiomyocytes. It contains one ETS DNA binding domain (aa 234-314). Within aa 125-231, mouse ETV2 shares 54% and 85% aa sequence identity with human and rat ETV2, respectively.

• Long Name:

  ETS Translocation 2

• Entrez Gene IDs:

  2116 (Human); 14008 (Mouse); 361544 (Rat)

• Alternate Names:

  ER71; ER71MGC129835; ETS translocation variant 2; ets variant 2; Ets-related protein 71; ETSRP71; ETSRP71ets variant gene 2; ETV2; MGC129834