• Species Reactivity

  Human

• Specificity

  Detects human Stabilin-1 in direct ELISAs.

• Source

  Monoclonal Mouse IgG    _2B Clone # 840449

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Stabilin-1    
  Gly2208-Val2305    
  Accession # Q9NY15

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Stabilin-1

Stabilin-1, also known as FEEL-1 (fasciclin, EGF-like, laminin type EGF-like, and link domain containing scavenger receptor-1) and CLEVER-1 (common lymphatic endothelial and vascular endothelial receptor-1), was originally identified as the MS-1 antigen. It is a type I transmembrane multi-domain protein with a large extracellular domain that contains 7 fasciclin domains, multiple EGF-like and laminin type EGF-like domains, and a link domain. The short cytoplasmic tail interacts with cargo adaptors that link cargo into the clathrin-coated pits (CGAs). Stabilin-1 is expressed by tissue macrophages and sinusoidal endothelial cells, which are scavenging cells. Stabilin-1 is involved in intracellular trafficking pathways and binds multiple ligands, including acetylated LDL, SPARC, AGE, and SI-CLP. An alternatively spliced, soluble, 803 amino acid (aa) Stabilin-1 that diverges after aa 746 has been identified. Within the region used as immunogen, human Stabilin-1 shares 83% and 84% amino acid sequence homology with mouse and canine Stabilin-1, respectively.

• Entrez Gene IDs:

  23166 (Human); 192187 (Mouse); 290559 (Rat)

• Alternate Names:

  CLEVER-1; fasciclin egf-like, laminin-type egf-like, and link domain-containing scavengerreceptor-1; Fasciclin, EGF-like, laminin-type EGF-like and link domain-containing scavengerreceptor 1; FEEL1; FEEL-1; FEEL-1MS-1 antigen; FELE-1; FEX1; KIAA0246common lymphatic endothelial and vascular endothelial receptor-1; MS-1 Antigen; STAB1; STAB-1; stabilin 1; Stabilin1; Stabilin-1