• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects human Inorganic Pyrophosphatase/PPA1 in direct ELISAs and human, mouse, and rat Inorganic Pyrophosphatase/PPA1 in Western blots.

• Source

  Monoclonal Mouse IgG    _2B Clone # 840941

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Inorganic Pyrophosphatase/PPA1    
  Met1-Asn289    
  Accession # Q15181

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Inorganic Pyrophosphatase/PPA1

PPA1 (Inorganic pyrophosphatase 1; also PPase and IOPPP) is a 32-36 kDa cytoplasmic member of the PPase family of enzymes. It is ubiquitously expressed, and acts on di- (or pyro) phosphate, generating orthophosphate in a Mg⁺-dependent manner. This activity can both generate energy for cells, or in the case of osteoblasts, provide raw material for calcification. The consumption of pyrophosphate may also remove inhibitors of enzymes such as guanylyl cyclase, and PPA1 itself is also reported to stimulate gene expression. Human PPA1 is 289 amino acids (aa) in length. There is one pyrophosphatase domain between aa 42-255, and two utilized acetylation sites at Lys 57 and Lys228. PPA1 is known to form homodimers. There is one potential alternative start site at Met46. Full-length human PPA1 shares 94% aa sequence identity with mouse PPA1.

• Entrez Gene IDs:

  5464 (Human); 852296 (Yeast)

• Alternate Names:

  diphosphate phosphohydrolase; EC 3.6.1.1; inorganic diphosphatase; inorganic pyrophosphatase 1; inorganic pyrophosphatase; IOPPP; IOPPPMGC111556; IPP1; PP1; PPA1; PPase; PPcytosolic inorganic pyrophosphatase; pyrophosphatase (inorganic) 1; pyrophosphatase (inorganic); pyrophosphatase 1; Pyrophosphate phospho-hydrolase; SID6-8061