• Species Reactivity

  Mouse

• Specificity

  Detects mouse GDF-8 propeptide in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) GDF‑1 propeptide, rmGDF-3 propeptide, rmGDF-5, or rmGDF-6 is observed.

• Source

  Monoclonal Rat IgG    _2B Clone # 84231

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse GDF-8    
  Asn25-Ser376    
  Accession # O08689

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Mouse GDF‑8/Myostatin Propeptide (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-8/Myostatin

Growth Differentiation Factor 8 (GDF-8), also known as Myostatin, is a secreted TGF-beta superfamily protein that is expressed specifically in developing and adult skeletal muscle. It controls myoblast proliferation and is a potent negative regulator of skeletal muscle mass (1‑3). Mouse GDF-8 is synthesized as a 376 amino acid (aa) preproprotein that consists of a 24 aa signal peptide, a 243 aa propeptide, and a 109 aa mature protein (2). Within the propeptide, mouse GDF-8 shares 96% and 99% aa sequence identity with human and rat GDF-8, respectively. GDF-8 is secreted as a preproprotein that is cleaved by BMP-1 family proteases to separate the 35‑40 kDa propeptide from the 12 kDa bioactive mature protein (4‑6). This results in a latent complex containing a disulfide-linked dimer of the mature protein and two noncovalently-associated molecules of the propeptide (2, 6). The GDF-8 propeptide functions as an inhibitor of mature GDF-8, and GDF-8 activity can also be inhibited through association with Follistatin, FLRG, Decorin, or GASP-1 (6‑11). The uncleaved GDF-8 proprotein binds Latent TGF-beta bp3 which can sequester it in the extracellular matrix and prevent the proteolytic cleavage of the propeptide (12). GDF-8 binds to the type II Activin receptor Activin RIIB which then associates with the type I receptors Activin RIB/ALK-4 or TGF-beta RI/ALK-5 to induce signaling (13). GDF-8 additionally inhibits adipogenic differentiation of mesenchymal stem cells and preadipocytes (14). Genetic deletion of GDF-8 or   in vivo administration of the GDF-8 propeptide induces muscle hypertrophy as well as enhanced glucose utilization and insulin sensitivity and a reduction in overall fat mass (15, 16).

• References:

1. McPherron, A.C. (2010) Immunol. Endocr. Metab. Agents Med. Chem. 10:217.

2. McPherron, A.C. et al. (1997) Nature 387:83.

3. Zimmers, T.A. et al. (2002) Science 296:1486.

4. Wolfman, N.M. et al. (2003) Proc. Natl. Acad. Sci. 100:15842.

5. McFarlane, C. et al. (2005) Dev. Biol. 283:58.

6. Lee, S.J. et al. (2001) Proc. Natl. Acad. Sci. 98:9306.

7. Thies, R.S. et al. (2001) Growth Factors 18:251.

8. Amthor, H. et al. (2004) Dev. Biol. 270:19.

9. Hill, J.J. et al. (2002) J. Biol. Chem. 277:40735.

10. Miura, T. et al. (2006) Biochem. Biophys. Res. Commun. 340:675.

11. Hill, J.J. et al. (2003) Molecular Endocrinology 17:1144.

12. Anderson, S.B. et al. (2008) J. Biol. Chem. 283:7027.

13. Rebbapragada, A. et al. (2003) Mol. Cell. Biol. 23:7230.

14. Guo, W. et al. (2008) J. Biol. Chem. 283:9136.

15. Matsakas, A. et al. (2009) Neuromuscul. Disord. 19:489.

16. Guo, T. et al. (2009) PloS ONE 4:e4937.

• Long Name:

  Growth Differentiation Factor 8

• Entrez Gene IDs:

  2660 (Human); 17700 (Mouse)

• Alternate Names:

  GDF8; GDF-8; GDF8growth differentiation factor 8; growth/differentiation factor 8; MSTN; Myostatin