• Species Reactivity

  Human

• Specificity

  Detects human Intelectin-2 in ELISA. In Direct ELISA, no cross-reactivity with recombinant human (rh) Intelectin-1, recombinant mouse (rm) Intelectin-1, or rmIntelectin-2 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 874029

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human Intelectin-2    
  Met1-Tyr324    
  Accession # Q8WWU7

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Intelectin-2

Intelectin-2 (ITLN-2; also endothelial lectin 2 and HL-2) is a 37-41 kDa, likely Ca-dependent carbohydrate-binding lectin that is a member of the X-lectin family of molecules.  It is secreted by intestinal Paneth cells, and appears to participate in fungal microbe recognition.  The human ITLN-2 precursor is 325 amino acids (aa) in length (SwissProt #:Q8WWU7).  It contains a 26 aa signal sequence and a 299 aa mature region.  Although mouse ITLN-2 is reportedly GPI-linked, and mouse and human ITLN-2 show considerable C-terminal aa identity, it is not known if human ITNL-2 is also GPI-linked.  There is one fibrinogen-related domain in the mature molecule (aa 44-267).  One potential splice variant is reported that shows a premature truncation after Gly264.  Over aa 29-324, human ITLN-2 shares 79% aa sequence identity with mouse ITLN-2/ITLN-1b, and 85% aa sequence identity with human ITLN-1.      

• Entrez Gene IDs:

  142683 (Human); 493583 (Mouse)

• Alternate Names:

  HL-2; intelectin 2; Intelectin2; Intelectin-2; Intelectin-b; Itln1b; ITLN2; Itlnb; UNQ2789/PRO7179