• Species Reactivity

  Human

• Specificity

  Detects human TACE/ADAM17 Cytosolic Domain in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human ADAM33 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 136133

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human TACE/ADAM17    
  Asp695-Cys824    
  Accession # P78536

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  HeLa human cervical epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # )

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Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TACE/ADAM17

TACE (also known as ADAM17) is a type I membrane zinc protease of the ADAM family that functions as a major sheddase on the cell surface.

• Long Name:

  TNF-alpha Converting Enzyme

• Entrez Gene IDs:

  6868 (Human); 11491 (Mouse)

• Alternate Names:

  ADAM 17; ADAM metallopeptidase domain 17; ADAM metallopeptidase domain 18; ADAM17; ADAM18; CD156b antigen; CD156b; CSVP; disintegrin and metalloproteinase domain-containing protein 17; EC 3.4.24.86; MGC71942; Snake venom-like protease; TACE; TACEcSVP; TNF-alpha convertase; TNF-alpha converting enzyme; TNF-alpha-converting enzyme; tumor necrosis factor, alpha, converting enzyme