• Species Reactivity

  Human

• Specificity

  Detects human Integrin alpha L/CD11a in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Integrin alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, alpha 6, alpha 8, alpha 9, alpha 10, alpha 11, alpha M, alpha V, alpha X, rhIntegrin beta 3, rhIntegrin alpha 6(x1)+ beta 4, alpha 7(x2)+ beta 1, alpha D+ beta 2, alpha E+ beta 7, recombinant mouse Integrin alpha 2b or alpha L is observed.    
   

• Source

  Monoclonal Mouse IgG2a Clone # CR38

• Immunogen

  Fibronectin purified human monocytes

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Human peripheral blood monocytes

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Integrin alpha L/CD11a

Integrin subunit alpha L/CD11a is a 180 kDa type I TM glycoprotein that interacts only with Integrin  beta 2/CD18 to form LFA-1, a leukocyte adhesion protein which binds endothelial cell ICAM. Human Integrin alpha L contains a 1064 aa extracellular domain (ECD), a 20 aa TM sequence and a 58 aa cytoplasmic domain. The ECD contains seven repeats that form a beta-propeller structure and one inserted vWA domain (I domain) containing a metal ion-dependent adhesion site (MIDAS). Human and mouse Integrin alpha L ECD share 74% aa identity. A second isoform has a 53 aa insert in the ECD.

• Entrez Gene IDs:

  3683 (Human); 16408 (Mouse); 308995 (Rat)

• Alternate Names:

  CD11 antigen-like family member A; CD11a antigen; CD11a; CD11Aantigen CD11A (p180), lymphocyte function-associated antigen 1, alphapolypeptide; Integrin alpha L; integrin alpha-L; integrin gene promoter; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen1; alpha polypeptide); ITGAL; Leukocyte adhesion glycoprotein LFA-1 alpha chain; Leukocyte function-associated molecule 1 alpha chain; LFA-1 alpha; LFA-1; LFA1A; LFA-1A; Ly-15; Ly-21; lymphocyte function-associated antigen 1