• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects human PP2C epsilon /PPPM1L in direct ELISAS and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human PP2C is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 700612

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human PP2C epsilon /PPPM1L    
  Asp43-Arg178    
  Accession # Q5SGD2

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.5 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PP2C epsilon/PPM1L

PP2C epsilon, also known as PPM1L, is a 34 kDa (predicted) widely expressed Ser/Thr phosphatase. It consists of a 25 amino acid (aa) extracellular domain, a 17 aa transmembrane segment, and a 318 aa cytoplasmic region (aa 43-360) which contains the phosphatase domain (aa 91-344). Alternate splicing generates additional isoforms of human PP2C epsilon with 179 aa or 127 aa N-terminal truncations or a 166 aa C-terminal truncation. The PP2C epsilon -mediated dephosphorylation of TAK1 and ASK1 is regulated by oxidative stress and inflammatory cytokines. Loss of PP2C epsilon function is associated with the development of metabolic syndrome. Within aa 43-178, human PP2C epsilon shares 99% aa sequence identity with mouse and rat PP2C epsilon.

• Long Name:

  Protein Phosphatase 2C alpha

• Entrez Gene IDs:

  151742 (Human); 242083 (Mouse); 310506 (Rat)

• Alternate Names:

  EC 3.1.3; EC 3.1.3.16; MGC132545; MGC132547; PP2C epsilon; PP2CE; PP2CEProtein phosphatase 2C isoform epsilon; PP2C-epsilon; PPM1L; PPM1-LIKE; protein phosphatase 1 (formerly 2C)-like; protein phosphatase 1L; Protein phosphatase 1-like; Protein phosphatase 2C epsilon isoform; protein phosphatase, Mg2+/Mn2+ dependent, 1L