• Species Reactivity

  Human

• Specificity

  Detects human Arylsulfatase B/ARSB in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ARSA, rhARSJ, or recombinant mouse ARSG is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 708401

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human Arylsulfatase B/ARSB    
  Ser37-Met533    
  Accession # P15848

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Arylsulfatase B/ARSB

Arylsulfatase B (ARSB) is a lysosomal enzyme of the sulfatase family that functions as an N-acetylgalactosamine-4-sulfatase (1). The enzyme is also active against chondroitin sulfate and dermatan sulfate (2). Deficiencies in ARSB activity are responsible for the lysosomal storage disease mucopolysaccharidosis Type VI, also known as Maroteaux-Lamy Syndrome (3).

• References:

1. Peters, C. et al. (1990) J. Biol. Chem. 265:3374.

2. O’Brien, J.S. et al. (1974) Biochem. Biophys. Res. Commun. 60:1170.

3. Wicker, G. et al. (1991) J. Biol. Chem. 266:21386.

• Entrez Gene IDs:

  411 (Human); 11881 (Mouse); 25227 (Rat)

• Alternate Names:

  ARSB; Arylsulfatase B; ASB; EC 3.1.6.12; G4S; MPS6; N-acetylgalactosamine-4-sulfatase