• Specificity

  Detects Streptavidin in Western blot.

• Source

  Recombinant Monoclonal Rabbit IgG Clone # 1220C

• Purification

  Protein A or G purified from cell culture supernatant

• Immunogen

  Purified streptavidin from     Streptomyces avidinii  

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.5 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Streptavidin

Streptavidin is a 52.8 kDa protein from the bacterium Streptomyces avidinii. Streptavidin is a homo-tetramer forming a beta-barrel with an extraordinarily high affinity for biotin (also known as vitamin B7). It has a dissociation constant (K  _d) on the order of ≈10  ⁻¹⁴ mol/L.   The binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature and has a high resistance to organic solvents, denaturants, detergents, proteolytic enzymes, and extreme pH ranges or temperatures.

• Alternate Names:

  SA V1; SA V2; Streptavidin V1; Streptavidin V2; Streptavidin