• Species Reactivity

  Mouse

• Specificity

  Detects mouse IGF-II in ELISAs and Western blots. In ELISAs, this antibody shows less than 6% cross-reactivity with recombinant human (rh) IGF‑I R and no cross‑reactivity with rhIGF-I and rmIGF-I is observed. In Western blots, 100% cross-reactivity with rhIGF‑II and no cross reactivity with rmIGF-I is observed.

• Source

  Monoclonal Rat IgG    _2A Clone # 122404

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant mouse IGF-II    
  Ala25-Glu91    
  Accession # P09535

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

• Mouse IGF-II Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 µg/mL 

  Mouse IGF‑II Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 µg/mL 

  Mouse IGF-II Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Mouse IGF-II Protein, CF (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-II

IGF-II (Insulin-like growth factor II; also multiplication-stimulating polypeptide/MSP and somatomedin-A) is a secreted 8 kDa polypeptide that belongs to the insulin family of peptide growth factors (1, 2, 3). It is part of a complex system of growth and metabolic-regulating proteins that is particularly important during development. It has been associated with nervous system proliferation and differentiation, myelination, adrenal cortical proliferation, and skeletal growth and differentiation (4). In human, IGF-II is primarily synthesized by the liver, and circulates at high levels in both fetus and adult. In rodent, however, IGF-II levels drop after the perinatal period, an effect attributed to the lack of a key gene promoter (2, 5). This may indicate that postnatally, IGF-II has either a limited, or local effect only in rodent. For example, evidence suggests IGF-II may be the intermediary for SHH induction of VEGF attendant with local neovascularization (6). Rodent cells known to express IGF-II include astrocytes (7), hepatocytes (8), osteoblasts (9), embryonic striated muscle cells (10, 11) plus Kupffer cells and Ito cells (12). Mouse IGF-II is synthesized as a 180 amino acid (aa) preproprecursor (13). It contains a 24 aa signal sequence, a 67 aa mature region, and an 89 aa C-terminal prodomain that is alternatively referred to as the E-peptide. Mature IGF-II is 91% and 97% aa identical to human and rat IGF-II, respectively. Proper processing of IGF-II requires the chaperone activity of GRP94 (14). This generates an 8 kDa mature form, an 18 kDa, 156 aa proform, and a potential 11 kDa, 88 aa “Big” form (aa 25 - 112). This 11 kDa ”Big” form would be equivalent to human 15 - 16 kDa IGF-II, with the 5 kDa difference attributable to the presence of O-linked glycosylation (15). There is an additional 34 aa proteolytic fragment that is termed preptin and contains aa 93 - 126 of the preproprecursor. This is distinct from IGF-II, is secreted by pancreatic B cells, and facilitates insulin secretion (16, 17). IGF-II has multiple binding partners. It binds to IGF-IR, the Insulin receptor (IR)-type A and IGF-IR:IR-A hybrids, the type 2 IGF receptor (IGF-2R), and IGF binding proteins 1 - 6 (18, 19). The first three receptors initiate downstream signaling events, the IGF-2R sequesters local IGF‑II, and the six IGFBPs regulate IGF-II activity in various tissues.

• References:

1. LeRoith, D. & C.T. Roberts Jr. (2003) Cancer Lett. 195:127.

2. Werner, H. & D. LeRoith (2000) Cell. Mol. Life Sci. 57:932.

3. Pavelic, J. et al. (2007) Indian J. Med. Res. 125:511.

4. Varela-Nieto, I. et al. (2007) Curr. Pharm. Des. 13:687.

5. Rotwein, P. & L.J. Hall (1990) DNA Cell Biol. 10:725.

6. Chao, W. & P.A. D-Amore (2008) Cytokine Growth Factor Rev. 19:111.

7. Rotwein, P. et al. (1988) Proc. Natl. Acad. Sci. USA 85:265.

8. Goya, L. et al. (1999) J. Biol. Chem. 274:24633.

9. McCarthy, T.L. et al. (1992) Endocrinology 130:1303.

10. Zindy, F. et al. (1992) J. Hepatol. 14:30.

11. Holthuizen, P.E. et al. (1993) Regul. Pept. 48:77.

12. Merrick, D. et al. (2007) BMC Dev. Biol. 7:65.

13. Stempien, M.M. et al. (1986) DNA 5:357.

14. Ostrovsky, O. et al. (2009) Mol. Biol. Cell 20:1855.

15. Daughaday, W.H. et al. (1993) Proc. Natl. Acad. Sci. USA 90:5823.

16. Buchanan, C.M. et al. (2001) Biochem. J. 360:431.

17. Cornish, J. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292:E117.

18. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.

19. Belfiore, A. (2007) Curr. Pharm. Des. 13:671.

• Long Name:

  Insulin-like Growth Factor II

• Entrez Gene IDs:

  3481 (Human); 16002 (Mouse)

• Alternate Names:

  C11orf43; chromosome 11 open reading frame 43; FLJ22066; FLJ44734; IGF2; IGF-2; IGFII; IGF-II; insulin-like growth factor 2 (somatomedin A); insulin-like growth factor II; insulin-like growth factor type 2; MSA; PP9974; somatomedin-A