• Specificity

  Detects N-terminal and C-terminal HA-tagged proteins in Western blot.

• Source

  Recombinant Monoclonal Rabbit IgG Clone # 1049F

• Purification

  Protein A or G purified from cell culture supernatant

• Immunogen

  HA Tag peptide    
  Accession # ABB51961

• Formulation

  Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  See below

• 
  

• Simple Western

  1 µg/mL

  See below

• 
  

• Immunoprecipitation

  1-4 µg/100 µg cell lysate

  HEK293 human embryonic kidney cell line transfected with C-terminal HA-tagged LRRN5 and N-terminal HA-tagged MICA, .

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C, as supplied.

• 1 month, 2 to 8 °C under sterile conditions after opening.

• 6 months, -20 to -70 °C under sterile conditions after opening.

Background: HA Tag

• Entrez Gene IDs:

  ABB51961.1 (Viral)

• Alternate Names:

  HA Epitope Tag; HA Tag; HA; Hemagglutinin