详细说明
Species Reactivity
Human
Specificity
Detects human EMMPRIN/CD147 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse EMMPRIN is observed.
Source
Monoclonal Mouse IgG 2B Clone # 109403
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human EMMPRIN/CD147
Ala22-His205
Accession # Q54A51Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Western Blot
0.2 µg/mL
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Western Blot | Detection of Human EMMPRIN/CD147 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue, human heart tissue, and human liver tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB972) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). A specific band was detected for EMMPRIN/CD147 at approximately |
Preparation and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EMMPRIN/CD147
EMMPRIN, also known as basigin and CD147, is a type I membrane protein belonging to the immunoglobulin superfamily. It is a multifunctional protein involved in reproduction, neural function, inflammation and tumor invasion.
Long Name:
Extracellular Matrix Metalloproteinase Inducer
Entrez Gene IDs:
682 (Human); 12215 (Mouse)
Alternate Names:
5F7; basigin (Ok blood group); Basigin; BSG; CD147 antigen; CD147; Collagenase stimulatory factor; EMMPRIN; EMMPRINTCSF; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M6; OK blood group antigen; OK; Tumor cell-derived collagenase stimulatory factor







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