• Species Reactivity

  Human

• Specificity

  Detects human B7-H7/HHLA2 in direct ELISAs.    
   

• Source

  Monoclonal Mouse IgG    ₁ Clone # 907812

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Human embryonic kidney cell line HEK293-derived human B7-H7/HHLA2    
  Met1-Asn344    
  Accession # Q9UM44

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Blockade of Receptor-ligand Interaction

  In a functional ELISA, 100-300 ng/mL of Mouse anti-Human B7-H7/HHLA2 Antibody (Catalog # MAB80841) will block 50% of the binding of 500 ng/mL of Recombinant Human B7-H7/HHLA2 Fc Chimera (Catalog # ) to immobilized Recombinant Human TMIGD2/CD28H Fc Chimera (Catalog # ) coated at 125 ng/mL    
  (100 µL/well). At 1.25 μg/mL, this antibody will block >90% of the binding.    
   

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-H7/HHLA2

B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins (1, 2). Mature human B7-H7 consists of a 322 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 49 aa cytoplasmic domain (3-5).  
B7‑H7 is constitutively expressed on monocytes and is up-regulated by LPS and IFN-gamma stimulation. It is expressed on LPS/IFN-gamma treated B cells but not on resting B cells (5). B7-H7 binds to cell surface determinants on resting and mature T cells, B cells, and monocytes as well as on immature and mature dendritic cells (5). Soluble B7-H7 inhibits the proliferation of activated CD4  ⁺ and CD8  ⁺ T cells and their production of IFN-gamma, TNF-alpha, IL-5, IL-10, IL-13, IL-17A, and IL-22 (5).

• References:

1. Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.

2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.

3. Mager, D.L. et al. (1999) Genomics 59:255.

4. Flajnik, M.M. et al. (2012) Immunogenetics 64:571.

5. Zhao, R. et al. (2013) Proc. Natl. Acad. Sci. USA 110:9879.

• Long Name:

  B7 Homolog 7

• Entrez Gene IDs:

  11148 (Human)

• Alternate Names:

  B7H7; B7-H7; HERV-H LTR-associating 2; HERV-H LTR-associating protein 2; HHLA2; Human endogenous retrovirus-H long terminal repeat-associating protein 2