• Species Reactivity

  Human

• Specificity

  Detects human ARNT/HIF‑1 beta in direct ELISAs.

• Source

  Monoclonal Mouse IgG    _2A Clone # 775146

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human ARNT/HIF‑1 beta    
  Asp517-Ala691    
  Accession # P27540

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ARNT/HIF-1 beta

ARNT (Aryl hydrocarbon nuclear translocator; also HIF-1 beta, Hypoxia-inducible factor 1-beta) is an 87-97 kDa member of the bHLH-PAS subclass of the bHLH family of transcription factors. It is widely expressed, and serves as part of both the NF kappa B and aryl hydrocarbon receptor (AHR) signaling nodes. For the AHR, the AHR is normally inactive and bound to hsp90 in the cytosol. Following ligand binding, the AHR complex enters the nucleus, dissociates from hsp90, dimerizes with ARNT, and then binds response elements of select genes. Human ARNT is 789 amino acids (aa) in length. It contains a DNA-binding region (aa 87‑143), two PAS domains (aa 161‑235 and 362‑458) and one PAC region (aa 424‑467). There are multiple potential splice forms. Two alternate start sites are found at Met10 and Met354, there is a deletion of aa 329‑755 and 77‑91, and an Ala substitution for aa 328‑789.

• Long Name:

  Aryl Hydrocarbon Receptor Nuclear Translocator

• Entrez Gene IDs:

  405 (Human); 11863 (Mouse); 25242 (Rat)

• Alternate Names:

  ARNT protein; ARNT; aryl hydrocarbon receptor nuclear translocator; BHLHE2; HIF1 beta; HIF-1 beta; HIF1B; HIF1BETA; HIF-1-beta; HIF1-beta; hypoxia-inducible factor 1, beta subunit; Hypoxia-inducible factor 1-beta; nuclear translocator; TANGO