• Species Reactivity

  Human

• Specificity

  Detects human A20 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human A20/TNFAIP3 (aa 440-790) is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 775912

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human A20    
  Lys91-Leu263    
  Accession # P21580

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: A20/TNFAIP3

A20 (TNF alpha -induced protein 3) is a cytoplasmic zinc finger protein that inhibits NF kappa B activity and tumor necrosis factor-mediated programmed cell death. The protein interacts with NAF1 and inhibits TNF-induced NF kappa B-dependent gene expression by interfering with RIP- or TRAF2-mediated transactivation signaling. A20 contains an N-terminal domain which has deubiquitinating enzyme activity and removes ubiquitin chains from receptor-interacting protein (RIP), thus mediating distinct regulatory effects in the down-regulation of NF kappa B signaling.

• Entrez Gene IDs:

  7128 (Human); 21929 (Mouse)

• Alternate Names:

  A20; A20TNFA1P2; EC 3.4.19.12; EC 6.3.2.-; MGC104522; MGC138687; OTU domain-containing protein 7C; OTUD7C; OTUD7CMGC138688; Putative DNA-binding protein A20; TNF alpha-induced protein 3; TNFA1P2; TNFAIP3; tumor necrosis factor alpha-induced protein 3; tumor necrosis factor inducible protein A20; tumor necrosis factor, alpha-induced protein 3; Zinc finger protein A20