• Species Reactivity

  Mouse

• Specificity

  Detects mouse BMP-5 in ELISA.

• Source

  Monoclonal Rat IgG    ₁ Clone # 781321

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant mouse BMP-5    
  Ala315-His452    
  Accession # P49003

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Neutralization

  Measured by its ability to neutralize BMP‑5-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Asahina, I.     et al. (1996) Exp. Cell Res. 222:38.The Neutralization Dose (ND    ₅₀) is typically 5-25 μg/mL in the presence of 2 μg/mL Recombinant Mouse BMP‑5.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.2 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-5

Bone Morphogenetic Protein-5 (BMP-5) is one of at least 15 structurally and functionally related BMPs which are members of the transforming growth factor  beta (TGF-beta ) superfamily (1). BMP-5 is synthesized as a 452 amino acid (aa) precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release the 20 kDa C-terminal mature protein (2, 3). Mature BMP-5 contains seven conserved cysteine residues involved in formation of the cysteine knot and the single interchain disulfide bond. Biologically active BMP-5 is a disulfide-linked homodimer of the C-terminal mature protein. Mature mouse BMP-5 shares 96% and 99% aa sequence identity with human and rat BMP-5, respectively. Cellular responses to BMP-5 are mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors (1). BMP-5 is expressed by chondrocytes in proliferating and hypertrophic zones of bone growth plates (4). It contributes to limb development by promoting proliferation and differentiation of chondrocytes as well as apoptosis of undifferentiated mesoderm (4, 5). Genetic defects in BMP-5 which cause C-terminal truncation or loss of the proteolytic cleavage site result in multiple skeletal abnormalities, including the   short ear phenotype in mice (3, 6). BMP-5 is also expressed by ovarian granulosa cells where it functions as an autocrine factor to promote GC proliferation and inhibit their production of progesterone (7). In the nervous system, BMP-5 promotes dendrite outgrowth and dopaminergic neuronal differentiation (8, 9). It is upregulated in oral squamous carcinoma cells and induces the apoptosis of some myeloma cell lines (10, 11).

• References:

1. Chen, D. et al. (2004) Growth Factors 22:233.

2. Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. 87:9843.

3. King, J.A. et al. (1994) Dev. Biol. 166:112.

4. Mailhot, G. et al. (2008) J. Cell. Physiol. 214:56.

5. Zuzarte-Luis, V. et al. (2004) Dev. Biol. 272:39.

6. Ho, A.M. et al. (2008) BMC Dev. Biol. 8:35.

7. Pierre, A. et al. (2005) Biol. Reprod. 73:1102.

8. Beck, H.N. et al. (2001) BMC Neurosci. 2:12.

9. Brederlau, A. et al. (2002) Mol. Cell. Neurosci. 21:367.

10. Jin, Y. et al. (2001) Oral Oncol. 37:225.

11. Ro, T.B. et al. (2004) Oncogene 23:3024.

• Long Name:

  Bone Morphogenetic Protein 5

• Entrez Gene IDs:

  653 (Human); 12160 (Mouse)

• Alternate Names:

  BMP5; BMP-5; bone morphogenetic protein 5; MGC34244