• Species Reactivity

  Human

• Specificity

  Detects human Secretin R in direct ELISAs and Western Blot.

• Source

  Monoclonal Mouse IgG    _2B Clone # 783526

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Secretin R delta 3/4Splice variant (1-3)    
  Gly65-Asn132    
  Accession # P47872

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Secretin R

SCTR (Secretin receptor) is a 51-64 kDa member of the G-protein coupled receptor 2 family. It is found on alveolar epithelium, bile duct epithelium, pancreatic exocrine duct epithelium, and stomach plus duodenal mucosal epithelium. Mature human SCTR is a 418 amino acid (aa) 7-transmembrane glycoprotein (aa 23-440). It contains a 121 aa N-terminal extracellular region (aa 23-143) plus a 48 aa C-terminal cytoplasmic domain (aa 393-440). There is at least one splice variant that shows a deletion of aa 66-101. SCTR forms homodimers and homooligomers, and heterodimerizes with almost all family B GPCRs (VPAC1; VPAC2; GLP1R; GHRHR; etc.). A splice form of the human Secretin Receptor with deletion of exons 3 and 4  was found expressed in pancreatic adenocarcinomas and cholangiocellular carcinomas, but not in gastrinomas or nonneoplastic pancreas or liver specimens.The deletion in this splice form is predicted to lead to a frame-shift, early truncation, and total absence of transmembrane segments in the receptor protein, whereas the leader sequence responsible for trafficking of the receptor to the cell membrane is preserved. This encoded a 111 aa peptide with its first 43 residues identical to wild-type receptor; but, subsequent to a shift in coding frame and early truncation, the next 68 residues were unique in the transcriptome/proteome. Our antibody was raised against this unique 68aa sequence resulting from putative frame-shift. 

• References:

1. Hayes, G.M. et al. (2007) Gastroenterology 133:853.

2. Korner, M. et al. (2005) Am. J. Pathol. 167:959.

3. Korner, M. et al. (2006) J. Hepatol. 45:825.

• Long Name:

  Secretin Receptor

• Entrez Gene IDs:

  6344 (Human); 319229 (Mouse); 81779 (Rat)

• Alternate Names:

  pancreatic secretin receptor; SCTR; SCT-R; Secretin R; secretin receptor; SecretinR; SR