• Species Reactivity

  Human

• Specificity

  Detects human Nodal in ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse Nodal is observed.

• Source

  Monoclonal Mouse IgG    _2A Clone # 784410

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Nodal    
  His238-Leu347    
  Accession # Q96S42

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nodal

Nodal is a secreted protein that is a member of the Transforming Growth Factor-beta (TGF-beta ) superfamily. Nodal was named for its localized expression in the mouse node during gastrulation, and is first detected in the early primitive streak during mesoderm formation. Expression of the Nodal gene occurs asymmetrically in the left, but not right, lateral plate during somitogenesis. Nodal proteins play crucial roles in mesoderm formation and both anterior-posterior and left-right axis formation during vertebrate development. Members of the Nodal gene family include mouse Nodal, chick cNR-1, frog Xnr1-4, and zebrafish cyclops. Biologically active Nodal is a disulfide-linked homodimer and contains all seven of the cysteine residues necessary for formation of the “cysteine knot” characteristic of TGF-beta -related molecules. Mouse Nodal is 34-39% homologous in the conserved region to other TGF-beta superfamily members. Nodal has been shown to signal through a mechanism related to the Activin pathway, and signaling is mediated through both Smad2 and 3. Nodal signaling utilizes type II Activin receptors, together with ALK4/ActRIB, or the type I receptor ALK7. Nodal interacts extracellularly with members of other protein families including Cerberus, Lefty, and EGF-CFC ligands such as Cripto. While the Cerberus and Lefty families act as Nodal antagonists, the EGF-CFC molecules act as co-receptors to facilitate Nodal signaling. The resulting concert of regulated Nodal activity allows for the precise control of mesoderm formation, neural patterning, and axis positioning and patterning during early vertebrate development.

• References:

1. Kumar, A. et al. (2001) J. Biol. Chem. 276:656.

2. Reissmann, E. et al. (2001) Genes & Dev. 15: 2010.

3. Schier, A. and M. Shen (1999) Nature 403:385.

4. Zhou, X. et al. (1993) Nature 361:543.

• Entrez Gene IDs:

  4838 (Human); 18119 (Mouse)

• Alternate Names:

  BMP-16; MGC138230; nodal homolog (mouse); nodal homolog; Nodal; nodal, mouse, homolog