• Species Reactivity

  Human

• Specificity

  Detects human ROR alpha /NR1F1 in direct ELISAs.    
   

• Source

  Monoclonal Mouse IgG    _2B Clone # 784652

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human ROR alpha /NR1F1    
  Arg47-His163    
  Accession # P35398

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Intracellular Staining by Flow Cytometry

  0.25 µg/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROR alpha/NR1F1

RORa (Retinoid-related Orphan Receptor alpha ) is a member of the NR1 nuclear hormone receptor family. ROR alpha is a DNA binding transcription factor, and can bind as a monomer or homodimer to hormone response elements to enhance expression.  Knockout mice implicate ROR alpha as being essential in the development of the cerebellum. It has also been reported to regulate lymphocyte development and play a role in Th17 differentiation. Human ROR alpha shares 99% amino sequence identity with mouse ROR alpha.

• Long Name:

  Retinoic Acid-Related Orphan Receptor alpha

• Entrez Gene IDs:

  6095 (Human)

• Alternate Names:

  DKFZp686M2414; MGC119326; NR1F1; NR1F1MGC119329; nuclear receptor ROR-alpha; Nuclear receptor RZR-alpha; Nuclear receptor subfamily 1 group F member 1; RAR-related orphan receptor A; RAR-related orphan receptor alpha; retinoic acid receptor-related orphan receptor alpha; retinoid-related orphan receptor alpha; Retinoid-related orphan receptor-alpha; ROR alpha; ROR1; ROR2; ROR3; RORA; RZRA; RZR-ALPHA; RZRAROR-alpha; transcription factor RZR-alpha