• Species Reactivity

  Human

• Specificity

  Detects human alpha 1‑Microglobulin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat alpha 1-Microglobulin is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 784917

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human alpha 1‑Microglobulin    
  Gly20-Val203    
  Accession # P02760

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha 1-Microglobulin

Human alpha 1-Microglobulin (alpha1-m/A1M; also protein HC) is a secreted, 31-32 kDa glycoprotein member of the lipocalin family, calycin superfamily of molecules. It is expressed by hepatocytes, keratinocytes, and endodermal derivatives in the embryo.  alpha 1-Microglobulin appears to act as a heme scavenger, protecting cells and collagen against oxidative damage. It also acts as an immunosuppressant, inhibiting polyclonal lymphocyte activation and dampening granulocyte migration in response to chemokines. alpha 1-Microglobulin circulates either as a monomer, or bound to IgA, albumin or prothrombin. Human alpha 1-Microglobulin is generated through cleavage of a precursor molecule termed AMBP. This AMBP should not be confused with AMBP-1, a 120-140 kDa adrenomedullin-binding protein that is also known as Complement Factor H. The AMBP precursor contains a 19 aa signal sequence, an N-terminal 183 aa alpha 1-Microglobulin protein (aa 20-203), and a C-terminal serine protease inhibitor termed bikunin (aa 206-352). alpha 1-Microglobulin possesses one lipocalin domain (aa 42-186). Although cleavage of AMBP in the Golgi apparatus typically generates a 31 kDa alpha 1-Microglobulin and 28 kDa bikunin molecule, the 60-65 kDa AMBP precursor can also be released intact.  alpha 1-Microglobulin will undergo extracellular processing, generating a 30 kDa isoform that is missing aa 199-203. There is one splice variant that shows a deletion of aa 48-57.  Over aa 20-203, human alpha 1-Microglobulin shares 76% aa sequence identity with both mouse and rat alpha 1-Microglobulin.

• Entrez Gene IDs:

  259 (Human); 11699 (Mouse); 25377 (Rat)

• Alternate Names:

  A1M; alpha 1Microglobulin; alpha 1-Microglobulin; alpha-1-microglobulin/bikunin precursor; AMBP; bikunin; EDC1; HCP; HCPcomplex-forming glycoprotein heterogeneous in charge; HI30; IATIL; inter-alpha-trypsin inhibitor light chain; ITI; ITIL; ITILC; ITILgrowth-inhibiting protein 19; protein AMBP; protein HC; trypstatin; uristatin; uronic-acid-rich protein; UTI