• Species Reactivity

  Human, Mouse

• Specificity

  Detects human PSMB7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human PSMB10 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 787807

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human PSMB7    
  Thr44-Ser277    
  Accession # Q99436

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.5 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PSMB7

PSMB7 (proteasome subunit, beta-type 7), also called proteasome subunit Z, is a widely expressed, non-catalytic component of the 20S proteasome core within the 26S proteasome that stabilizes late stages of proteasome assembly. Human PSMB7 is synthesized as a 277 amino acid (aa) protein with a 43 aa propeptide and a 234 active protein that contains one potential tyrosine phosphorylation site. Active human PSMB7 shares 97% aa sequence identity with mouse and rat PSMB7. Total PSMB7 expression is positively correlated with chemotherapy resistance in human breast cancer.

• Long Name:

  Proteasome (Prosome, Macropain) Subunit, beta Type, 7

• Entrez Gene IDs:

  5695 (Human); 19177 (Mouse); 85492 (Rat)

• Alternate Names:

  Macropain Chain Z; Proteasome Subunit Z; PSMB7